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Proteintech anti mgp
Anti Mgp, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mgp/MGP+Antibody/pm41714535-61-47-49
Average 94 stars, based on 42 article reviews

anti mgp - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: MGP + and IDO1 + tumor-associated macrophages facilitate immunoresistance in breast cancer revealed by single-cell RNA sequencing.
Article Snippet: Immunotherapy is widely applied for the treatment of breast cancer, but to which some patients respond poorly or develop resistance.. Therefore, the mechanism needs to be further studied.. Transcriptomic data of 31 breast cancer patients treated with anti-programmed death receptor 1 (PD-1) was downloaded from the VIB-KULeuven Center for Cancer Biology to analyze the changes in myeloid cells in tumor tissues before and after immunotherapy.

Article Title: The regenerating skeletal muscle niche drives satellite cell return to quiescence
Article Snippet: EdU incorporation was visualized using the Click-iT EdU Alexa Fluor 488 imaging kit (ThermoFisher) following the manufacturer’s protocol. .. Primary antibodies [anti-Pax7, 2 μg/mL (Developmental Studies Hybridoma Bank (DSHB) at the University of Iowa), anti-mgp 1:50 (Proteintech), anti-ncoa7 1:50 (Novus Biologicals), anti-mest 1:100 (Invitrogen), anti-itm2a 1:50 (Proteintech), anti-HO-1 1:100 (Proteintech), anti-gabarap1 1:50 (abcam), anti-parD3 1:200 (Santa Cruz Biotechnology), anti-phospho histone 3 1:500 (Millipore)] were incubated with intact myofibers at room temperature for 1hour followed by three washes in PBS. .. Myofibers were then incubated with appropriate fluorescently conjugated secondary antibodies [Donkey anti-mouse IgG1 (ThermoFisher), anti-rat (ThermoFisher), anti-goat (ThermoFisher), anti-mouse IgG2a (ThermoFisher)] diluted 1:1000 for 1hour at room temperature.

Article Title: Persistent Gene Activation as a Molecular Signature of Ulcerative Colitis Progression to Colorectal Cancer.
Article Snippet: Introduction Ulcerative colitis (UC) is an inflammatory disease characterized by colonic epithelial damage.. With prolonged disease duration, the risk of malignant transformation increases significantly.. However, the mechanisms driving the progression from chronic inflammation to tumorigenesis remain incompletely understood.

Article Title: MGP regulates the adipogenic differentiation of mesenchymal stem cells in osteoporosis via the Ca2+/CaMKII/RIP140/FABP3 axis
Article Snippet: .. For MGP IHC staining, the sections were subjected to antigen retrieval, blocked in 5% goat serum, and then incubated with anti-MGP (Proteintech 60055-1-Ig). ..

Article Title: MGP regulates the adipogenic differentiation of mesenchymal stem cells in osteoporosis via the Ca2+/CaMKII/RIP140/FABP3 axis.
Article Snippet: .. For MGP IHC staining, the sections were subjected to antigen retrieval, blocked in 5% goat serum, and then incubated with anti-MGP (Proteintech 60055-1-Ig). ..

Activity Assay:

Blocking Assay:

Binding Assay:

other:

Article Title: Complement Regulatory Protein CD46 Manifests a Unique Role in Promoting the Migration of Bladder Cancer Cells
Article Snippet: CD46 antibodies were obtained from OriGene Technologies, Inc. (Rockville, MD, USA); antibodies against MGP, AFAP1-AS1, and TGFB1 were from Proteintech (Rosemont, IL, USA); MT1G from Lifespan Biosciences (Shirley, MA, USA); KRT13, GABRP, and C3-α chain from Abcam (Seoul, Korea); SIGLEC6 from R&D systems (Minneapolis, MN, USA); and CYP24A1 and β-actin antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Article Title: The regenerating skeletal muscle niche drives satellite cell return to quiescence
Article Snippet: anti-mgp , Proteintech , Cat# 10734-1-AP, RRID:AB_2297660.

Immunohistochemistry:

Staining:

Image Search Results

a Experimental setup for the in vitro experiments. Cells were seeded at Day 0 (D0), CM was generated on D1 to D2 (24 hours) and receiving cells were exposed on D2 to D4 (48 h). b Venn diagrams showing the number of upregulated and downregulated genes obtained by RNA-seq of CM-exposed LECs. c Heatmap showing the top DEGs in the CM-exposed LECs. Gray triangles indicate the result of unsupervised clustering for genes and cell types behaving in a similar way. d Volcano plots depicting genes upregulated and downregulated in LECs after CM exposure (two-sided Wald test). Genes indicated in red are considered mostly pro-inflammatory, whereas genes in blue are anti-inflammatory. e Pathway analysis showing the most significantly downregulated and upregulated pathways (the integrated two-sided Fisher’s exact test). LECs are from 4 different sources ( n = 4) ( a – e ). f Gene expression changes in CM-exposed LECs ( n = 3–9 HLECs). Data were analyzed with a one-way ANOVA. g Protein expression levels of MGP in LECs following CM exposure ( n = 6 HLECs). Data were analyzed with a one-way ANOVA linear mixed model and Dunnett’s correction. h Protein expression of MGP and podoplanin on LECs after coculture of T47D cells with LECs compared to culture with CM. Data were analyzed with two-way ANOVA and Sidak’s multiple comparison test ( n = 4 HLECs). i mRNA Expression of CDH2 , CDH5 , and SNAI1 in CM-exposed LECs. Data were shown as box plots ( n = 5–7 HLECs). Data were analyzed using a two-way ANOVA linear mixed model and statistical significance was adjusted with Dunnett multiplicity correction. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Journal: Nature Communications

Article Title: Breast cancer remodels lymphatics in sentinel lymph nodes

doi: 10.1038/s41467-025-64981-z

Figure Lengend Snippet: a Experimental setup for the in vitro experiments. Cells were seeded at Day 0 (D0), CM was generated on D1 to D2 (24 hours) and receiving cells were exposed on D2 to D4 (48 h). b Venn diagrams showing the number of upregulated and downregulated genes obtained by RNA-seq of CM-exposed LECs. c Heatmap showing the top DEGs in the CM-exposed LECs. Gray triangles indicate the result of unsupervised clustering for genes and cell types behaving in a similar way. d Volcano plots depicting genes upregulated and downregulated in LECs after CM exposure (two-sided Wald test). Genes indicated in red are considered mostly pro-inflammatory, whereas genes in blue are anti-inflammatory. e Pathway analysis showing the most significantly downregulated and upregulated pathways (the integrated two-sided Fisher’s exact test). LECs are from 4 different sources ( n = 4) ( a – e ). f Gene expression changes in CM-exposed LECs ( n = 3–9 HLECs). Data were analyzed with a one-way ANOVA. g Protein expression levels of MGP in LECs following CM exposure ( n = 6 HLECs). Data were analyzed with a one-way ANOVA linear mixed model and Dunnett’s correction. h Protein expression of MGP and podoplanin on LECs after coculture of T47D cells with LECs compared to culture with CM. Data were analyzed with two-way ANOVA and Sidak’s multiple comparison test ( n = 4 HLECs). i mRNA Expression of CDH2 , CDH5 , and SNAI1 in CM-exposed LECs. Data were shown as box plots ( n = 5–7 HLECs). Data were analyzed using a two-way ANOVA linear mixed model and statistical significance was adjusted with Dunnett multiplicity correction. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Article Snippet: Cells were blocked for 20 min with human Ig on ice, permeabilized with the BD Cytofix/Cytoperm Kit (554714), and sequentially stained for 30 min with the following antibodies: MGP (NBP2-45844) from Novus (1:150); negative control, mouse IgG2a (553454) from BD and anti-mouse IgG2a AF546 (A21133) from Invitrogen.

Techniques: In Vitro, Generated, RNA Sequencing, Gene Expression, Expressing, Comparison, Cell Culture

a Expression of MGP in LECs after direct exposure to recombinant VEGF165 (5, 25, or 100 ng/mL), VEGF-C (5, 50, or 100 ng/mL), TGF-β (5, 25, or 100 ng/mL), or EGF (5, 25, or 100 ng/mL) are shown ( n = 4 HLECs). Data were depicted as Tukey box plots and analyzed using one-way ANOVA linear mixed models (Sidak correction) fitted separately for each parameter with group (recombinant vs control), dose and their interaction as fixed effects. b Gene expression changes in modified CM-exposed LECs are shown as determined by qPCR. CM was generated in the presence of antibodies against VEGFR3 (1 or 10 μg/mL, n = 4 HLECs), TGF-β (2 or 20 μg/mL, n = 8–10 HLECs), and EGF (1 or 10 μg/mL, n = 3–5 HLECs), compared to isotype control exposed samples and data were depicted as Tukey box plots showing relative gene changes and analyzed using a one-way ANOVA linear mixed models (Sidak) fitted separately for each parameter with group (antibody vs control) and dose and their interaction as fixed effects. c MGP expression determined by qPCR. CM generated with culture media devoid of VEGF supplement was used. Data were shown as Tukey box plots ( n = 9 HLECs). Data were analyzed using a one-way ANOVA linear mixed model (Sidak). The center line of the box plots represents the median, the box the 25th to 75th percentiles and the whiskers the inner fences. Statistics of group and dose effects are presented within the boxes; significant differences in comparison to the controls (defined as 1) are indicated by p values. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Journal: Nature Communications

Article Title: Breast cancer remodels lymphatics in sentinel lymph nodes

doi: 10.1038/s41467-025-64981-z

Figure Lengend Snippet: a Expression of MGP in LECs after direct exposure to recombinant VEGF165 (5, 25, or 100 ng/mL), VEGF-C (5, 50, or 100 ng/mL), TGF-β (5, 25, or 100 ng/mL), or EGF (5, 25, or 100 ng/mL) are shown ( n = 4 HLECs). Data were depicted as Tukey box plots and analyzed using one-way ANOVA linear mixed models (Sidak correction) fitted separately for each parameter with group (recombinant vs control), dose and their interaction as fixed effects. b Gene expression changes in modified CM-exposed LECs are shown as determined by qPCR. CM was generated in the presence of antibodies against VEGFR3 (1 or 10 μg/mL, n = 4 HLECs), TGF-β (2 or 20 μg/mL, n = 8–10 HLECs), and EGF (1 or 10 μg/mL, n = 3–5 HLECs), compared to isotype control exposed samples and data were depicted as Tukey box plots showing relative gene changes and analyzed using a one-way ANOVA linear mixed models (Sidak) fitted separately for each parameter with group (antibody vs control) and dose and their interaction as fixed effects. c MGP expression determined by qPCR. CM generated with culture media devoid of VEGF supplement was used. Data were shown as Tukey box plots ( n = 9 HLECs). Data were analyzed using a one-way ANOVA linear mixed model (Sidak). The center line of the box plots represents the median, the box the 25th to 75th percentiles and the whiskers the inner fences. Statistics of group and dose effects are presented within the boxes; significant differences in comparison to the controls (defined as 1) are indicated by p values. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Techniques: Expressing, Recombinant, Control, Gene Expression, Modification, Generated, Comparison, Cell Culture

a MGP expression in LECs after CM treatment with anti-MGP antibody ( n = 5–6 HLECs), analyzed using one-way ANOVA linear mixed models (Sidak correction). b MGP expression in LECs exposed to anti-MGP antibody or recombinant MGP ( n = 6–10 HLECs), analyzed using two-way ANOVA linear mixed models (Sidak correction). c MGP expression in siRNA-silenced LECs ( n = 5 HLECs), analyzed by two-sided Mann–Whitney U -test. d Tube formation quantified by number of nodes, junctions and branches in MGP-silenced vs. control LECs, shown as geometric mean with 95% CI ( n = 17 HLECs), analyzed with a two-sided paired t -test. e Scratch assay of MGP-silenced and control LECs over 2 days (mean ± SEM; n = 13 HLECs), analyzed by repeated measures two-way ANOVA (matched full mixed model) with Sidak’s multiple comparisons. f Soluble MGP binding to cancer cells: MGP (His) was added with anti-His antibody (red), while the control (blue) contained only cancer cells with antibody. Histograms represent two independent experiments. g Ex vivo adhesion assay of T47D cells binding to lymphatic sinuses of six metastatic and five non-metastatic LNs from four and five patients, respectively (two-sided ratio paired t -test). LN sections were treated with anti-MGP or control antibody. The binding after control antibody was defined as 100% due to day-to-day variation. Example images show T47D cells binding after control vs. anti-MGP treatment to the same metastatic LN area. Adherent cells (some marked by yellow arrowheads) lie on top of tissue sections, focus adjusted to highlight cell adhesion. Scale bar: 50 μm. For box plots: center line = median; box = 25th–75th percentiles; whiskers = inner fences. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Journal: Nature Communications

Article Title: Breast cancer remodels lymphatics in sentinel lymph nodes

doi: 10.1038/s41467-025-64981-z

Figure Lengend Snippet: a MGP expression in LECs after CM treatment with anti-MGP antibody ( n = 5–6 HLECs), analyzed using one-way ANOVA linear mixed models (Sidak correction). b MGP expression in LECs exposed to anti-MGP antibody or recombinant MGP ( n = 6–10 HLECs), analyzed using two-way ANOVA linear mixed models (Sidak correction). c MGP expression in siRNA-silenced LECs ( n = 5 HLECs), analyzed by two-sided Mann–Whitney U -test. d Tube formation quantified by number of nodes, junctions and branches in MGP-silenced vs. control LECs, shown as geometric mean with 95% CI ( n = 17 HLECs), analyzed with a two-sided paired t -test. e Scratch assay of MGP-silenced and control LECs over 2 days (mean ± SEM; n = 13 HLECs), analyzed by repeated measures two-way ANOVA (matched full mixed model) with Sidak’s multiple comparisons. f Soluble MGP binding to cancer cells: MGP (His) was added with anti-His antibody (red), while the control (blue) contained only cancer cells with antibody. Histograms represent two independent experiments. g Ex vivo adhesion assay of T47D cells binding to lymphatic sinuses of six metastatic and five non-metastatic LNs from four and five patients, respectively (two-sided ratio paired t -test). LN sections were treated with anti-MGP or control antibody. The binding after control antibody was defined as 100% due to day-to-day variation. Example images show T47D cells binding after control vs. anti-MGP treatment to the same metastatic LN area. Adherent cells (some marked by yellow arrowheads) lie on top of tissue sections, focus adjusted to highlight cell adhesion. Scale bar: 50 μm. For box plots: center line = median; box = 25th–75th percentiles; whiskers = inner fences. Source data, non-significant p values and detailed experiment and n -numbers (biologically independent samples of cultured cells) are provided in the Source Data file.

Techniques: Expressing, Recombinant, MANN-WHITNEY, Control, Wound Healing Assay, Binding Assay, Ex Vivo, Cell Adhesion Assay, Cell Culture

a Differential abundance testing using MiloR and a heatmap of differentially expressed genes between differential abundance neighborhoods in LN LECs. The UMAP plot was shown in Fig. and is inserted here for clarity. In the heatmap, columns and rows represent neighborhoods and differentially expressed genes, respectively. Expression values for each gene are scaled between 0 and 1. The upper panel of the heatmap shows the neighborhood log fold change. FDR, false discovery rate. b Heatmap showing the expression of the top DEGs between metastatic and distant LNs for each cell. Bars above the heatmap indicate the tissue and cluster origin of each cell (LNs, clusters). c Violin plots displaying the top DEG expression between metastatic and distant LNs by cluster, with log-normalized expression value labeled. Nine patients’ samples were integrated for this analysis ( a – c ). d Immunostaining of MGP and its quantification in metastatic and distant LNs. Zoomed-in images show SCS and medullary sinuses containing cancer cells and MGP expression on PROX1 + LECs (arrows). Blue, cytokeratin; red, MGP; green, PROX1. Scale bars, 125 μm. Images are representative of four individuals with similar results. Circles in the bar plots represent biological replicates (mean ± SEM, two-tailed, unpaired Student’s t -test). Metastatic LN, n = 4 patients; non-metastatic LN, n = 4 patients. Source data are provided as a Source Data file. e MGP expression in LECs of non-metastatic and metastatic LNs detected using Visium HD. f Gene Ontology (GO) enrichment analysis of the top DEGs (Wilcoxon rank-sum test, metastatic vs. distant LNs) using the EnrichR package. FDR-corrected p values are shown.

Journal: Nature Communications

Article Title: Breast cancer remodels lymphatics in sentinel lymph nodes

doi: 10.1038/s41467-025-64981-z

Figure Lengend Snippet: a Differential abundance testing using MiloR and a heatmap of differentially expressed genes between differential abundance neighborhoods in LN LECs. The UMAP plot was shown in Fig. and is inserted here for clarity. In the heatmap, columns and rows represent neighborhoods and differentially expressed genes, respectively. Expression values for each gene are scaled between 0 and 1. The upper panel of the heatmap shows the neighborhood log fold change. FDR, false discovery rate. b Heatmap showing the expression of the top DEGs between metastatic and distant LNs for each cell. Bars above the heatmap indicate the tissue and cluster origin of each cell (LNs, clusters). c Violin plots displaying the top DEG expression between metastatic and distant LNs by cluster, with log-normalized expression value labeled. Nine patients’ samples were integrated for this analysis ( a – c ). d Immunostaining of MGP and its quantification in metastatic and distant LNs. Zoomed-in images show SCS and medullary sinuses containing cancer cells and MGP expression on PROX1 + LECs (arrows). Blue, cytokeratin; red, MGP; green, PROX1. Scale bars, 125 μm. Images are representative of four individuals with similar results. Circles in the bar plots represent biological replicates (mean ± SEM, two-tailed, unpaired Student’s t -test). Metastatic LN, n = 4 patients; non-metastatic LN, n = 4 patients. Source data are provided as a Source Data file. e MGP expression in LECs of non-metastatic and metastatic LNs detected using Visium HD. f Gene Ontology (GO) enrichment analysis of the top DEGs (Wilcoxon rank-sum test, metastatic vs. distant LNs) using the EnrichR package. FDR-corrected p values are shown.

Article Snippet: Briefly, non-metastatic and metastatic LN sections were treated with anti-MGP antibody (1:100, Proteintech 10734-1-AP) or a negative control antibody (Rabbit IgG 1:100, Proteintech 30000-0-A) for 30 min.

Techniques: Expressing, Labeling, Immunostaining, Two Tailed Test

Journal: Nature Communications

Article Title: Breast cancer remodels lymphatics in sentinel lymph nodes

doi: 10.1038/s41467-025-64981-z

Techniques: Expressing, Recombinant, MANN-WHITNEY, Control, Wound Healing Assay, Binding Assay, Ex Vivo, Cell Adhesion Assay, Cell Culture

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anti mgp (Proteintech)

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